Determination of the reference genes in Streptococcus equi subsp. zooepidemicus for real-time PCR
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F15%3APU114977" target="_blank" >RIV/00216305:26310/15:PU114977 - isvavai.cz</a>
Alternative codes found
RIV/00216305:26310/15:PU117615
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Determination of the reference genes in Streptococcus equi subsp. zooepidemicus for real-time PCR
Original language description
The whole genome expression analysis of Streptococcus equi subsp. zooepidemicus strain ATCC 35246 was performed using DNA microarrays. According to the stability of gene expression 10 genes including gyrA and gyrB were chosen as potential reference genes. Further analysis of expression stability of selected genes was done by real-time PCR. Ct values from PCR analysis were processed in Microsoft Excel and our conclusions were additionally confirmed by GeNorm normalization. Reference gene was chosen
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů