Detection of Lactobacillus acidophilus species in the probiotic meat product

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F15%3APU115346" target="_blank" >RIV/00216305:26310/15:PU115346 - isvavai.cz</a>
Alternative codes found
RIV/00216305:26310/15:PU122752
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Detection of Lactobacillus acidophilus species in the probiotic meat product
Original language description
Probiotic bacteria have beneficial effects on human health. They positively influence the host immune system and the composition of intestinal microflora. They can reach the gastrointestinal tract with food. Probiotic bacteria are found in dairy productsand food supplements most often. They can be used in other matrices including meat products. The aim of this study was to detect Lactobacillus acidophilus cells in probiotic fermented meat product ?Ovčácká klobása? using the PCR method. Attention was paid to the preparation of the sample (homogenization, lysis of cells) and optimization of methods for the isolation of DNA in quality suitable for PCR using magnetic microparticles. The use of magnetic particles with immobilized antibody antiLactobacilluswas also tested for Lactobacillius acidophilus bacterial cells isolation from homogenized product. Bacterial cells bound on the magnetic particles were identified using PCR. Specific PCR products for the species Lactobacillus acidophilus
Czech name
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Czech description
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Project
<a href="/en/project/GP13-32840P" target="_blank" >GP13-32840P: The development of encapsulation magnetic nano- and microparticles using real time polymerase chain reaction</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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