G-quadruplex-based structural transitions in 15-mer DNA oligonucleotides varying in lengths of internal oligo(dG) stretches detected by voltammetric techniques
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F21%3APU143827" target="_blank" >RIV/00216305:26620/21:PU143827 - isvavai.cz</a>
Alternative codes found
RIV/68081707:_____/15:00456709 RIV/00216224:14740/15:00083572
Result on the web
<a href="https://www.scopus.com/record/display.uri?eid=2-s2.0-84952826168&origin=inward&txGid=daf30a0832d4ac7af6b4e6142afa8380" target="_blank" >https://www.scopus.com/record/display.uri?eid=2-s2.0-84952826168&origin=inward&txGid=daf30a0832d4ac7af6b4e6142afa8380</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00216-015-8768-1" target="_blank" >10.1007/s00216-015-8768-1</a>
Alternative languages
Result language
angličtina
Original language name
G-quadruplex-based structural transitions in 15-mer DNA oligonucleotides varying in lengths of internal oligo(dG) stretches detected by voltammetric techniques
Original language description
Electrochemical methods, particularly when applied in connection with mercury-containing electrodes, are excellent tools for studying nucleic acids structure and monitoring structural transitions. We studied the effect of the length of the central (dG)n stretch (varying from 0 to 15 guanine residues) in 15-mer oligodeoxynucleotides (ODN, G0 to G15) on their electrochemical and interfacial behavior at mercury and carbon electrodes. The intensity of guanine oxidation signal at the carbon electrode (peak Gox) was observed to increase continuously with number of guanines between 0 and 15, with only a slight positive shift for ODNs with seven or more guanines in the central segment. Very different effects were observed when the peak GHMDE was measured at the mercury electrode. Intensity of the latter signal increased with number of guanines up to G5, and decreased sharply with further elongation of the (dG)n stretch. CD spectroscopy and electrophoresis experiments revealed formation of parallel intermolecular quadruplex structures for ODNs containing five or more G residues. Further measurements made by cyclic and alternating-current voltammetry revealed a strong influence of the ODN structure on their behavior at electrically charged surfaces. © 2015 Springer-Verlag Berlin Heidelberg
Czech name
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Czech description
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Classification
Type
J<sub>SC</sub> - Article in a specialist periodical, which is included in the SCOPUS database
CEP classification
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OECD FORD branch
10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
Result continuities
Project
<a href="/en/project/GAP206%2F12%2F2378" target="_blank" >GAP206/12/2378: Influence of conformation and chemical modification on the behavior of oligonucleotides at electrodes: towards the development of DNA sensors</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytical and Bioanalytical Chemistry
ISSN
1618-2642
e-ISSN
1618-2650
Volume of the periodical
407
Issue of the periodical within the volume
12
Country of publishing house
DE - GERMANY
Number of pages
10
Pages from-to
5817-5826
UT code for WoS article
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EID of the result in the Scopus database
2-s2.0-84952826168