Significant discrepancy in cyclosporin A post-dose concetrations when analyzed with specific RIA and HPLC method

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00843989%3A_____%2F10%3A00100920" target="_blank" >RIV/00843989:_____/10:00100920 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Significant discrepancy in cyclosporin A post-dose concetrations when analyzed with specific RIA and HPLC method

Original language description

C(2) or AUC sparse sampling methods are widely recommended for therapeutic monitoring of cyclosporin A (CsA). One additional reason for promoting the C(2) sampling time in place of commonly used C(0) is that the C(2) level may actually provide more accurate measurement of parent drug concentration by immunoassays, as lower portion of metabolites has been formed 2 hours post-dose than at the steady-state trough time point. HPLC and RIA whole blood levels of CsA and its main metabolites AM1, AM9 and AWN were compared during 12 hours profile after chronic administration. 40 stable renal transplant male patients (age 49 +/- 6 years, body weight 76 +/- 7 kg) were treated with CsA (Sandimmun Neoral(R), Novartis s.r.o, Prague, Czech Republic) in doses 198 +/-56 mg twice daily. Samples were collected in steady state (after 2 weeks of regular treatment regimen) as follows: pre-dose, 0.5, 1, 1.5, 2, 3, 5, 8 and 12 hours after dose. CsA concentrations were determined both specific RIA assay (Cyc

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FR - Pharmacology and apothecary chemistry

OECD FORD branch

—

Project

<a href="/en/project/1A8655" target="_blank" >1A8655: New Possibilities of TDM of Cyclosporine A and its Metabolites after kidney transplantation</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International journal of clinical pharmacology and therapeutics

ISSN

0946-1965

e-ISSN

—

Volume of the periodical

48

Issue of the periodical within the volume

2

Country of publishing house

DE - GERMANY

Number of pages

6

Pages from-to

87-92

UT code for WoS article

000274770100001

EID of the result in the Scopus database

—