The pentafluorophenyl stationary phase shows a unique separation efficiency for performing fast chromatography determination of highbush blueberry anthocyanins

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F17%3AN0000022" target="_blank" >RIV/25271121:_____/17:N0000022 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11160/17:10362222

Result on the web

<a href="http://dx.doi.org/10.1016/j.talanta.2017.01.061" target="_blank" >http://dx.doi.org/10.1016/j.talanta.2017.01.061</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.talanta.2017.01.061" target="_blank" >10.1016/j.talanta.2017.01.061</a>

Result language

angličtina

Original language name

The pentafluorophenyl stationary phase shows a unique separation efficiency for performing fast chromatography determination of highbush blueberry anthocyanins

Original language description

A high-performance liquid chromatography method using an alternative pentafluorophenyl (PFP) core-shell stationary phase has been developed and used for rapid separation of 23 anthocyanins in a highbush blueberry Bluehaven cultivar. A high efficiency of separation of anthocyanins was achieved in the core-shell column Kinetex PFP, 150×4.6 mm (particle size 2.6 μm) with a 5×4.6 mm precolumn, using a simple linear gradient elution with a mobile phase of acetonitrile and a water solution of 2% formic acid at a flow rate of 1.0 ml/min and at a temperature of 50 °C. The detection wavelength was set at 520 nm for detection of all anthocyanins. The homogenized blueberry sample (Bluehaven cultivar) was extracted using pure methanol with 1.3% formic acid using an ultrasound bath for 20 min and then filtrated. A 5-μL sample volume was directly injected into the HPLC system. The developed method showed an efficient separation of 23 anthocyanins in a total runtime of 21 min. The potential of the pentafluorophenyl phase for efficient separation was demonstrated on a wide range of anthocyanins varying in glycosylation and acylation patterns found in highbush blueberries. The fluorinated stationary phase showed an alternative and complementary separation approach providing unique aromatic and polar selectivity in comparison with common C-18 phases.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10611 - Plant sciences, botany

Project

<a href="/en/project/LO1608" target="_blank" >LO1608: Research Pomological Centre</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Talanta

ISSN

0039-9140

e-ISSN

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Volume of the periodical

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Issue of the periodical within the volume

166

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

6

Pages from-to

249-254

UT code for WoS article

000395839700034

EID of the result in the Scopus database

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