Whey substrates enriched by substances supporting nisine production

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26722861%3A_____%2F17%3AN0000074" target="_blank" >RIV/26722861:_____/17:N0000074 - isvavai.cz</a>

Result on the web

<a href="http://www.biomicroworld2017.org/files/bookofabstracts.pdf" target="_blank" >http://www.biomicroworld2017.org/files/bookofabstracts.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Whey substrates enriched by substances supporting nisine production

Original language description

Nisin is known for its antimicrobial activity against many pathogenic microorganisms. Due to this reason, it is used as a food preservative approved by World Health Organization. The aim of this study was to assemble an optimal substrate composition for maximal nisin production. Reconstituted sweet whey and demineralized whey were used as a base mediums. These base mediums were enriched by combinations of protein and carbohydrate components (2 % peptone, soya peptone or casein hydrolysate, 5 % and 10 % lactose or maltodextrin). Nisin production was monitored by agar diffusion method. Lactococcus lactis subsp. lactis CCDM 731 was selected as a nisin-producing strain and Micrococcus luteus ATCC 10240 was used as an indicator of growth inhibition. The nisin production was higher in all enriched mediums compared to the basal whey mediums. Based on our results, it is obvious that fortified demineralized whey medium seems to be more suitable substrate for nisin production than fortified sweet whey medium. Our results also suggest that fortification of basal whey substrates by combination of protein and carbohydrate supplements significantly increases nisin production. Casein hydrolysate and maltodextrin were the most suitable combination for fortification of both sweet whey and demineralized whey medium.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

40101 - Agriculture

Project

<a href="/en/project/QJ1310254" target="_blank" >QJ1310254: Research into the use of whey as dairy industry waste product, the production of antimicrobial compounds for the modification of hydrophilic polymer systems with the use in cosmetic and medical applications</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů