Novel identification methods including a species-specific PCR for hazardous Bacillus species

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26722861%3A_____%2F19%3AN0000048" target="_blank" >RIV/26722861:_____/19:N0000048 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22330/19:43919316

Result on the web

<a href="https://www.akademiai.com/doi/10.1556/066.2019.48.4.2" target="_blank" >https://www.akademiai.com/doi/10.1556/066.2019.48.4.2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1556/066.2019.48.4.2" target="_blank" >10.1556/066.2019.48.4.2</a>

Result language

angličtina

Original language name

Novel identification methods including a species-specific PCR for hazardous Bacillus species

Original language description

Fifteen strains of Bacillus spp. (three B. cereus strains, one B. subtilis, B. macerans, B. pumilus, and B. mycoides strains, and eight unknown isolates from the food-industry) were identified on species level with three designed pairs of primers for B. cereus, B. licheniformis, and B. subtilis. Primers designed for B. licheniformis (BlichF and BlichR) and B. subtilis (BsubF and BsubR) allowed specific species identification, whereas the designed pair of primers for B. cereus (BcerF2 and BcerR2) showed specificity for B. cereus sensu lato, because both B. cereus strains and B. mycoides gave positive reaction. Bacilli identification was also carried out with routine API method with unsatisfactory results. The (GTG)5-PCR method was used for strain characterization, enabling bacilli classification into separate clusters according to their taxonomic designations.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

40500 - Other agricultural sciences

Project

<a href="/en/project/QJ1210300" target="_blank" >QJ1210300: Protection systems of quality and safety of dairy products by means of suitable methods applicable in practice</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Alimentaria

ISSN

0139-3006

e-ISSN

1588-2535

Volume of the periodical

48

Issue of the periodical within the volume

4

Country of publishing house

HU - HUNGARY

Number of pages

8

Pages from-to

415 – 422

UT code for WoS article

000499075600002

EID of the result in the Scopus database

2-s2.0-85079223523