Estimation of the putrescine and cadaverine production in E.coli strains using the PCR methods

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F49608851%3A_____%2F10%3A%230000405" target="_blank" >RIV/49608851:_____/10:#0000405 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Estimation of the putrescine and cadaverine production in E.coli strains using the PCR methods
Original language description
In our study, the ornithine (speC) and lysine (cadA) decarboxylase genes were investigated in E. coli strains using the PCR methods. 26 E. coli strains isolated from raw cow, sheep and goat bulk milk samples and cheeses were evaluated. The alignment of the multiple sequences and analysis of conserved domains in the studied genes was carried out using ClustalW and BLAST programs. The conserved ODC (ornithine decarboxylase like family) domain was selected as a target for primer design of the both genes. Analysis of the sequence sources for the speC gene revealed existence of speC isosyme, featured with homology between the 66 - 98 % comparising to the speC nucleotide alignment. Also, in the case of cadA gene, we observed two groups of sequences with thehomology of approximately 60% (GenBank, cadA1, cadA2). On the basement of these facts, we designed and performed also additional PCR tests. 81 % strains were positive for speC, 75 % for speC isozyme gene, 54 % for cadA1 and 45 % for cadA2
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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