Raw Goat's Milk, Fresh and Soft Cheeses as a Potential Source of Encephalitozoon cuniculi
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F21%3A43903534" target="_blank" >RIV/60076658:12220/21:43903534 - isvavai.cz</a>
Alternative codes found
RIV/60077344:_____/21:00555214
Result on the web
<a href="https://pubmed.ncbi.nlm.nih.gov/33978456/" target="_blank" >https://pubmed.ncbi.nlm.nih.gov/33978456/</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1089/fpd.2021.0017" target="_blank" >10.1089/fpd.2021.0017</a>
Alternative languages
Result language
angličtina
Original language name
Raw Goat's Milk, Fresh and Soft Cheeses as a Potential Source of Encephalitozoon cuniculi
Original language description
The aim of this work was to evaluate the effect of pasteurization and coagulation during goat cheese production on the infectivity of Encephalitozoon cuniculi spores for immunodeficient (SCID, CD4(-/-), and CD8(-/-)) and immunocompetent (BALB/c and C57BL/6) mice. Goat milk and fecal samples were screened for the presence and quantity of microsporidial DNA using molecular methods. Experimentally produced cheese from E. cuniculi-enriched goat milk or goat cheese purchased from retail producers was fed with experimental mice susceptible to E. cuniculi infection. The mice were sacrificed in the presumed acute phase of infection and samples of their tissues were subject to molecular detection of specific E. cuniculi DNA. Specific DNA of E. cuniculi genotype II was detected in feces and milk of three out of 99 goats kept on 6 farms in the Czech Republic. Under experimental conditions, spores of E. cuniculi genotype II remained viable in artificially enriched fresh cheese and were able to cause infection in laboratory mice. E. cuniculi genotype I and II DNA were detected in eight of the nine goat cheeses purchased from various producers/breeders in the Czech Republic and these cheeses were able to develop infection in both immunodeficient and immunocompetent mice. The results of these experiments showed that spores of E. cuniculi genotype I and II are able to remain viable after cheese processing and thus fresh and soft cheeses should be considered a potential source of microsporidia.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40301 - Veterinary science
Result continuities
Project
<a href="/en/project/GA20-10706S" target="_blank" >GA20-10706S: The Trojan Horse of the immune system: Do microsporidia exploit migrating immune cells for their own dispersal in the host body?</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Foodborne Pathogens and Disease
ISSN
1535-3141
e-ISSN
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Volume of the periodical
18
Issue of the periodical within the volume
9
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
661-667
UT code for WoS article
000649417300001
EID of the result in the Scopus database
2-s2.0-85113291620