Comprehensive sub-mitochondrial protein map of the parasitic protist Trypanosoma brucei defines critical features of organellar biology
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F23%3A00576576" target="_blank" >RIV/60077344:_____/23:00576576 - isvavai.cz</a>
Alternative codes found
RIV/61988987:17310/23:A2402MYI RIV/60076658:12310/23:43906684
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S221112472301094X?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S221112472301094X?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.celrep.2023.113083" target="_blank" >10.1016/j.celrep.2023.113083</a>
Alternative languages
Result language
angličtina
Original language name
Comprehensive sub-mitochondrial protein map of the parasitic protist Trypanosoma brucei defines critical features of organellar biology
Original language description
We have generated a high-confidence mitochondrial proteome (MitoTag) of the Trypanosoma brucei procy-clic stage containing 1,239 proteins. For 337 of these, a mitochondrial localization had not been described before. We use the TrypTag dataset as a foundation and take advantage of the properties of the fluorescent protein tag that causes aberrant but fortuitous accumulation of tagged matrix and inner membrane proteins near the kinetoplast (mitochondrial DNA). Combined with transmembrane domain predictions, this charac-teristic allowed categorization of 1,053 proteins into mitochondrial sub-compartments, the detection of unique matrix-localized fucose and methionine synthesis, and the identification of new kinetoplast proteins, which showed kinetoplast-linked pyrimidine synthesis. Moreover, disruption of targeting signals by tagging allowed mapping of the mode of protein targeting to these sub-compartments, identifying a set of C-tail anchored outer mitochondrial membrane proteins and mitochondrial carriers likely employing multiple target peptides. This dataset represents a comprehensive, updated mapping of the mitochondrion.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Cell Reports
ISSN
2211-1247
e-ISSN
2211-1247
Volume of the periodical
42
Issue of the periodical within the volume
9
Country of publishing house
US - UNITED STATES
Number of pages
19
Pages from-to
113083
UT code for WoS article
001073362600001
EID of the result in the Scopus database
2-s2.0-85169920202