Tepsin and AP4 mediate transport from the trans-Golgi to the plant-like vacuole in toxoplasma
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60077344%3A_____%2F25%3A00640500" target="_blank" >RIV/60077344:_____/25:00640500 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/25:10504490
Result on the web
<a href="https://doi.org/10.1083/jcb.202312109" target="_blank" >https://doi.org/10.1083/jcb.202312109</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1083/jcb.202312109" target="_blank" >10.1083/jcb.202312109</a>
Alternative languages
Result language
angličtina
Original language name
Tepsin and AP4 mediate transport from the trans-Golgi to the plant-like vacuole in toxoplasma
Original language description
Apicomplexan parasites are obligate intracellular pathogens possessing unique organelles but lacking several components of the membrane trafficking machinery conserved in other eukaryotes. While some of these components have been lost during evolution, others remain undetectable by standard bioinformatics approaches. Using a conditional splitCas9 system in Toxoplasma gondii, we previously identified TGGT1_301410, a hypothetical gene conserved among apicomplexans, as a potential trafficking factor. Here, we show that TGGT1_301410 is a distant ortholog of T. gondii tepsin (TgTEP), localized to the trans-Golgi and functioning as an accessory protein of the adaptor protein complex 4 (AP4). We demonstrate that AP4-TgTEP is essential for the actin-dependent transport of vesicles to the plant-like vacuole (PLVAC) and Golgi organization. Notably, our findings reveal that, unlike in metazoans, the AP4 complex in T. gondii utilizes clathrin as a coat protein, a mechanism more closely aligned with that of plants. These results underscore a conserved yet functionally adapted vesicular transport system in Apicomplexa.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Cell Biology
ISSN
0021-9525
e-ISSN
1540-8140
Volume of the periodical
224
Issue of the periodical within the volume
12
Country of publishing house
US - UNITED STATES
Number of pages
29
Pages from-to
e202312109
UT code for WoS article
001592568700001
EID of the result in the Scopus database
2-s2.0-105018648559