Introduction to protoplast culture of Allium genus
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60109807%3A_____%2F12%3A%230000224" target="_blank" >RIV/60109807:_____/12:#0000224 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Introduction to protoplast culture of Allium genus
Original language description
The aim of this study was to establish the protoplast culture of Allium genus and verify the influence of electric field on regeneration ability of isolated protoplasts. Another goal was tested the possibilities of somatic hybridization in this genus. Mesophyll protoplasts were isolated from in vitro cultured plants of Allium flavum, A. fistulosum, A. schoenoprasum and A. ursinum. The protoplasts were isolated and fused using established protocol for Solanum genus: http://www.vubhb.cz/_te.asp?f=institute/06dogr-protocols-mfpep.htm with prolonged time of enzyme action. The induction of regeneration ability was tested using five parameters of electric field. The regeneration ability of affected protoplasts and fused protoplasts (A. flavum + A. ursinum; A. fistulosum + A. ursinum; A. fistulosum + A. schoenoprasum) was compared with non-affected control. All cultivated protoplasts maintained viability. The cell wall regeneration and cell division were observed in different time and intensi
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
EI - Biotechnology and bionics
OECD FORD branch
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Result continuities
Project
<a href="/en/project/LF11012" target="_blank" >LF11012: Biotechnological production process of Allium ursinum for food- and pharmaceutical industry.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů