Preliminariy Experientce with Protoplast Culture of Allium ursinum.

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60109807%3A_____%2F15%3A%230000351" target="_blank" >RIV/60109807:_____/15:#0000351 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Preliminariy Experientce with Protoplast Culture of Allium ursinum.
Original language description
Somatic hybridization is a technique for alternative breeding to increase genetic variability. The aim of this work was to establish protoplast culture of Allium ursinum. The initial genotypes originated from three localities. The homogeneity of these populations was tested using RAPD markers. These analyses showed some differences among tested genotypes. According to preliminary experiments, Alliumursinumprotoplasts seemed to be recalcitrant for protoplast cultures. The influence of plantgrow regulators, sugar type and electric field (AC, DC and combination) was tested.With some modifications, the protoplasts were isolated and affected by electric field according toGreplova et al. (2008). The application of indirect current seemed to be useful tostimulate cell division.Sugar type in culture medium appeared to be very important; the combination of glucose with sucrose proved to be the best.Putrescine showed to be crucial for cell viability and regeneration.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GE - Plant cultivation
OECD FORD branch
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Project
<a href="/en/project/LF11012" target="_blank" >LF11012: Biotechnological production process of Allium ursinum for food- and pharmaceutical industry.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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