Pralidoxime - the gold standard of acetylcholinesterase reactivators - reactivation efficacy in vitro

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F10%3A00002398" target="_blank" >RIV/60162694:G44__/10:00002398 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Pralidoxime - the gold standard of acetylcholinesterase reactivators - reactivation efficacy in vitro
Original language description
In this work we aim to summarize the universality of this compound, its reactivatiom potency when different cholinesterase inhibitors are used. Background: Pralidoxime is considered as a gold standard of acetylcholinesterase reactivators - antidotes usedin case of nerve agent poisonings. It is commercially available worldwide since many years back. However, several studies describe this oxime as an old-fashion antidote Methods: Pralidoxime was synthesized at our department. The reactivating efficacy was tested on 10% (w/v) rat brain homogenate that was incubated with appropriate inhibitor for 30 minutes to reach 96% inhibition of AChE. Then, pralidoxime was added for 10 minutes. Measurements were performed at 25 °C, pH 8, and at the concentrations ofthe AChE reactivators at 10-3 and 10-5 M. The activities of brain AChE were measured by a potentiostatic method. Results: No sufficient reactivation was achieved at the concentration 10-5 M, which is a concentration that is possible to re
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FP - Other medical fields
OECD FORD branch
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Project
<a href="/en/project/ME%20865" target="_blank" >ME 865: Countermeasure against chemical terrorism - development of new antidotes against nerve agents</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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