Simple detection of primary cilia by immunofluorescence
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F20%3A00555795" target="_blank" >RIV/60162694:G44__/20:00555795 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/20:10425140 RIV/00216208:11150/20:10425140 RIV/00179906:_____/20:10425140 RIV/00064190:_____/20:N0000070
Result on the web
<a href="https://www.jove.com/video/61155/simple-detection-of-primary-cilia-by-immunofluorescence" target="_blank" >https://www.jove.com/video/61155/simple-detection-of-primary-cilia-by-immunofluorescence</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3791/61155" target="_blank" >10.3791/61155</a>
Alternative languages
Result language
angličtina
Original language name
Simple detection of primary cilia by immunofluorescence
Original language description
Primary cilia are dynamically regulated during cell cycle progression, specifically during the G0/G1 phases of the cell cycle, being resorbed prior to mitosis. Primary cilia can be visualized with highly sophisticated methods, including transmission electron microscopy, 3D imaging, or using software for the automatic detection of primary cilia. However, immunofluorescent staining of primary cilia is needed to perform these methods. This publication describes a protocol for the easy detection of primary cilia in vitro by staining acetylated alpha tubulin (axoneme) and gamma tubulin (basal body). This immunofluorescent staining protocol is relatively simple and results in high-quality images. The present protocol describes how four cell lines (C2C12, MEF, NHLF, and skin fibroblasts) expressing primary cilia were fixed, immunostained, and imaged with a fluorescent or confocal microscope.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10700 - Other natural sciences
Result continuities
Project
—
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Jove-Journal of Visualized Experiments
ISSN
1940-087X
e-ISSN
1940-087X
Volume of the periodical
2020
Issue of the periodical within the volume
159
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
e61155
UT code for WoS article
000546406600049
EID of the result in the Scopus database
2-s2.0-85085362508