Using proteomics to identify host cell interaction partners for VgrG and IglJ

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F20%3A00556188" target="_blank" >RIV/60162694:G44__/20:00556188 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11160/20:10417508

Result on the web

<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7471685/" target="_blank" >https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7471685/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-020-71641-3" target="_blank" >10.1038/s41598-020-71641-3</a>

Result language

angličtina

Original language name

Using proteomics to identify host cell interaction partners for VgrG and IglJ

Original language description

Francisella tularensis is a highly virulent intracellular bacterium and the causative agent of tularemia. The disease is characterized by the suboptimal innate immune response and consequently by the impaired adaptive immunity. The virulence of this pathogen depends on proteins encoded by a genomic island termed the Francisella Pathogenicity Island (FPI). However, the precise biological roles of most of the FPI-encoded proteins remain to be clarified. In this study, we employed stable isotope labeling by amino acids in cell culture (SILAC) in combination with affinity protein purification coupled with liquid chromatography-mass spectrometry to identify potential protein-effector binding pairs for two FPI virulence effectors IglJ and VgrG. Our results may indicate that while the IglJ protein interactions primarily affect mitochondria, the VgrG interactions affect phagosome and/or autophagosome biogenesis via targeting components of the host's exocyst complex.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10700 - Other natural sciences

Project

—

Continuities

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Scientific Reports

ISSN

2045-2322

e-ISSN

—

Volume of the periodical

10

Issue of the periodical within the volume

1

Country of publishing house

DE - GERMANY

Number of pages

9

Pages from-to

14612

UT code for WoS article

000571229700104

EID of the result in the Scopus database

2-s2.0-85090166789