Activation of protein kinase C suppresses fragmentation of pig oocytes aged in vitro
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F11%3A50748" target="_blank" >RIV/60460709:41210/11:50748 - isvavai.cz</a>
Alternative codes found
RIV/00027014:_____/11:#0001389
Result on the web
<a href="http://dx.doi.org/10.1017/S1751731110002119" target="_blank" >http://dx.doi.org/10.1017/S1751731110002119</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1017/S1751731110002119" target="_blank" >10.1017/S1751731110002119</a>
Alternative languages
Result language
angličtina
Original language name
Activation of protein kinase C suppresses fragmentation of pig oocytes aged in vitro
Original language description
When cultured for an extended time, pig oocytes which matured in vitro to the stage of metaphase II undergo the complex process designated as ageing. Activation of protein kinases C (PKCs) using phorbol-12-myristate-13-acetate (PMA) protects oocytes fromfragmentation. None oocyte was fragmented after 3 days of ageing in 50 nM of PMA. A similar effect (8 % of fragmented oocytes) was observed after 3 day treatment of ageing oocytes with 100 ?M of 1-stearoyl-2arachidonoyl-sn-glycerol (STEAR). PMA and STEAR activate both calcium-dependent and calcium-independent PKCs. This combined effect on PKCs seems to be essential for the protection of oocytes from fragmentation. Neither the specific activator of calcium-dependent PKCs 1-oleoyl-2-acetyl-sn-glycerol (OLE) nor specific activator of calcium-independent PKCs dipalmitoyl-L-alpha-phosphatidylinositol-3,4,5-triphosphate heptaammonium salt (DIPALM) suppressed fragmentation of ageing pig oocytes.
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GG - Zootechnics
OECD FORD branch
—
Result continuities
Project
<a href="/en/project/QG50052" target="_blank" >QG50052: Reproductive biotechniques in pigs</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Animal
ISSN
1751-7311
e-ISSN
—
Volume of the periodical
5
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
565-571
UT code for WoS article
000288130800009
EID of the result in the Scopus database
—