CREB-binding protein plays key roles in juvenile hormone action in the red flour beetle, Tribolium Castaneum

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41320%2F18%3A78771" target="_blank" >RIV/60460709:41320/18:78771 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1038/s41598-018-19667-6" target="_blank" >http://dx.doi.org/10.1038/s41598-018-19667-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-018-19667-6" target="_blank" >10.1038/s41598-018-19667-6</a>

Result language

angličtina

Original language name

CREB-binding protein plays key roles in juvenile hormone action in the red flour beetle, Tribolium Castaneum

Original language description

Juvenile hormones (JH) and ecdysteroids regulate many biological and metabolic processes. CREB-binding protein (CBP) is a transcriptional co-regulator with histone acetyltransferase (HAT) activity. Therefore, CBP is involved in activation of many transcription factors that regulate expression of genes associated with postembryonic development in insects. However, the function of CBP in JH action in insects is not well understood. Hence, we studied the role of CBP in JH action in the red flour beetle, Tribolium castaneum and the Tribolium cell line. CBP knockdown caused a decrease in JH induction of genes, Kr-h1, 4EBP and G13402 in T. castaneum larvae, adults and TcA cells whereas, Trichostatin A TSA, a histone deacetylase (HDAC) inhibitor induced the expression of these JH-response genes. Western blot analysis with specific antibodies revealed the requirement of CBP for the acetylation of H3K18 and H3K27 in both T. castaneum and TcA cells. Chromatin immunoprecipitation (Chip) assays showed the import

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

—

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Scientific Reports

ISSN

2045-2322

e-ISSN

—

Volume of the periodical

8

Issue of the periodical within the volume

JAN2018

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

1-11

UT code for WoS article

000423044400042

EID of the result in the Scopus database

2-s2.0-85041068110