Determination of the maximum operating range of hydrodynamic stress in mammalian cell culture

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F15%3A43900188" target="_blank" >RIV/60461373:22340/15:43900188 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.jbiotec.2014.12.003" target="_blank" >http://dx.doi.org/10.1016/j.jbiotec.2014.12.003</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jbiotec.2014.12.003" target="_blank" >10.1016/j.jbiotec.2014.12.003</a>

Result language
angličtina
Original language name
Determination of the maximum operating range of hydrodynamic stress in mammalian cell culture
Original language description
Application of quality by design (QbD) requires identification of the maximum operating range for parameters affecting the cell culture process. These include hydrodynamic stress, mass transfer or gradients in dissolved oxygen and pH. Since most of theseare affected by the impeller design and speed, the main goal of this work was to identify a maximum operating range for hydrodynamic stress, where no variation of cell growth, productivity and product quality can be ensured. Two scale-down models were developed operating under laminar and turbulent condition, generating repetitive oscillating hydrodynamic stress with maximum stress values ranging from 0.4 to 420 Pa, to compare the effect of the different flow regimes on the cells behavior. Two manufacturing cell lines (CHO and Sp2/0) used for the synthesis of therapeutic proteins were employed in this study. For both cell lines multiple process outputs were used to determine the threshold values of hydrodynamic stress, such as cell gro
Czech name
—
Czech description
—

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CI - Industrial chemistry and chemical engineering
OECD FORD branch
—

Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)