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Base-modified DNA labeled by [Ru(bpy)3]2+ and [Os(bpy)3]2+ complexes: construction by polymerase incorporation of modified nucleoside triphosphates, electrochemical and luminescent properties, and applications

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F09%3A00324504" target="_blank" >RIV/61388963:_____/09:00324504 - isvavai.cz</a>

  • Alternative codes found

    RIV/68081707:_____/09:00324504

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Base-modified DNA labeled by [Ru(bpy)3]2+ and [Os(bpy)3]2+ complexes: construction by polymerase incorporation of modified nucleoside triphosphates, electrochemical and luminescent properties, and applications

  • Original language description

    Modified nucleoside triphosphates bearing [Ru (bpy)3]2+ and [Os(bpy)3]2+ complexes attached via acetylene linker were prepared by cross-coupling reactions and were further used for incorporation to DNA by polymerase. The metal complexes were used as luminescent or redox label for detection of DNA. Multi-color redox labeling of DNA by four different labels was also developed.

  • Czech name

    DNA obsahující modifikované báze označené [Ru (bpy)3]2+ a [Os(bpy)3]2+ komplexy: konstrukce inkorporacemi modifikovaných nukleosid trofosfátů polymerasou, elektrochemické a luminiscenční vlastnosti a aplikace

  • Czech description

    Modifikované nukleosid trifosfáty nesoucí [Ru (bpy)3]2+ a [Os(bpy)3]2+ komplexy připojené přes acetylenový můstek byly připraveny cross-coupling reakcemi a byly dále použity pro inkorporace do DNA katalyzované polymerasou. Komplexy kovů byly potom využity jako luminiscenční nebo redoxní značky pro detekci DNA. Vícebarevné kódování DNA bylo rovněž vyvinuto s použitím 4 různých redoxních značek.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CC - Organic chemistry

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2009

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Chemistry - A European Journal

  • ISSN

    0947-6539

  • e-ISSN

  • Volume of the periodical

    15

  • Issue of the periodical within the volume

    5

  • Country of publishing house

    DE - GERMANY

  • Number of pages

    11

  • Pages from-to

  • UT code for WoS article

    000263093700012

  • EID of the result in the Scopus database