Transient and Switchable (Triethylsilyl)ethynyl Protection of DNA against Cleavage by Restriction Endonucleases

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F11%3A00364215" target="_blank" >RIV/61388963:_____/11:00364215 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1002/anie.201102898" target="_blank" >http://dx.doi.org/10.1002/anie.201102898</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/anie.201102898" target="_blank" >10.1002/anie.201102898</a>

Result language
angličtina
Original language name
Transient and Switchable (Triethylsilyl)ethynyl Protection of DNA against Cleavage by Restriction Endonucleases
Original language description
The first transient chemical protection of DNA against RE cleavage was developed. (Triethylsilyl)ethynyl-modified 7-deaza-dATP is readily incorporated to DNA by primer extension or PCR using KOD XL polymerase. The silylethynyl-protected DNA resists the cleavage by restriction endonucleases (REs). After treatment with NH3, the deprotected DNA is fully cleavable by the REs.
Czech name
—
Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CC - Organic chemistry
OECD FORD branch
—

Project
<a href="/en/project/GA203%2F09%2F0317" target="_blank" >GA203/09/0317: Construction of novel functional nucleic acids for applications in chemical biology, catalysis and self assembly.</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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