Flexible double-headed cytosine-linked 2'-deoxycytidine nucleotides. Synthesis, polymerase incorporation to DNA and interaction with DNA methyltransferases
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F16%3A00458981" target="_blank" >RIV/61388963:_____/16:00458981 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/16:10323538
Result on the web
<a href="http://dx.doi.org/10.1016/j.bmc.2016.01.057" target="_blank" >http://dx.doi.org/10.1016/j.bmc.2016.01.057</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bmc.2016.01.057" target="_blank" >10.1016/j.bmc.2016.01.057</a>
Alternative languages
Result language
angličtina
Original language name
Flexible double-headed cytosine-linked 2'-deoxycytidine nucleotides. Synthesis, polymerase incorporation to DNA and interaction with DNA methyltransferases
Original language description
New types of double-headed 2'-deoxycytidine 5'-0-triphosphates (dOcTPs) bearing another cytosine or 5-fluorocytosine linked through a flexible propargyl, homopropargyl or pent-1-ynyl linker to position 5 were prepared by the aqueous Sonogashira cross -coupling reactions of 5-iodo-dCTP with the corresponding (fluoro)cytosine-alkynes. The modified dCxcTPs were good substrates for DNA polymerases and were used for enzymatic synthesis of cytosine-functionalized DNA by primer extension or PCR. The cytosine or fluorocytosine-linked DNA probes did not significantly inhibit DNA methyltransferases and did not cross-link to these proteins.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CC - Organic chemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GBP206%2F12%2FG151" target="_blank" >GBP206/12/G151: Center of novel approaches to bioanalysis and molecular diagnostics</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Bioorganic & Medicinal Chemistry
ISSN
0968-0896
e-ISSN
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Volume of the periodical
24
Issue of the periodical within the volume
6
Country of publishing house
GB - UNITED KINGDOM
Number of pages
9
Pages from-to
1268-1276
UT code for WoS article
000370822700012
EID of the result in the Scopus database
2-s2.0-84958889792