LC/MS analysis and deep sequencing reveal the accurate RNA composition in the HIV-1 virion

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F19%3A00507271" target="_blank" >RIV/61388963:_____/19:00507271 - isvavai.cz</a>
Alternative codes found
RIV/68378050:_____/19:00522861 RIV/00216208:11110/19:10395894 RIV/60461373:22310/19:43919275
Result on the web
<a href="https://www.nature.com/articles/s41598-019-45079-1" target="_blank" >https://www.nature.com/articles/s41598-019-45079-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-019-45079-1" target="_blank" >10.1038/s41598-019-45079-1</a>

Result language
angličtina
Original language name
LC/MS analysis and deep sequencing reveal the accurate RNA composition in the HIV-1 virion
Original language description
The mechanism of action of various viruses has been the primary focus of many studies.Yet, the data on RNA modifications in any type of virus are scarce. Methods for the sensitive analysis of RNA modifications have been developed only recently and they have not been applied to viruses. In particular, the RNA composition of HIV-1 virions has never been determined with sufficiently exact methods. Here, we reveal that the RNA of HIV-1 virions contains surprisingly high amount of the 1-methyladenosine. We are the first to use a liquid chromatography-mass spectrometry analysis (LC/MS) of virion RNA, which we combined with nil-A profiling and deep sequencing. We found that m(1)A was present in the tRNA, but not in the genomic HIV-1 RNA and the abundant 7SL RNA. We were able to calculate that an HIV-1 virion contains per 2 copies of genomic RNA and 14 copies of 7SL RNA also 770 copies of tRNA, which is approximately 10 times more than thus far expected. These new insights into the composition of the HIV-1 virion can help in future studies to identify the role of nonprimer tRNAs in retroviruses. Moreover, we present a promising new tool for studying the compositions of virions.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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