Honeybee Iflaviruses Pack Specific tRNA Fragments from Host Cells in Their Virions
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F22%3A00559536" target="_blank" >RIV/61388963:_____/22:00559536 - isvavai.cz</a>
Alternative codes found
RIV/68378050:_____/22:00559536 RIV/60461373:22310/22:43925547 RIV/60076658:12310/22:43904907 RIV/00216208:11110/22:10445458 RIV/00216224:14740/22:00128475
Result on the web
<a href="https://doi.org/10.1002/cbic.202200281" target="_blank" >https://doi.org/10.1002/cbic.202200281</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/cbic.202200281" target="_blank" >10.1002/cbic.202200281</a>
Alternative languages
Result language
angličtina
Original language name
Honeybee Iflaviruses Pack Specific tRNA Fragments from Host Cells in Their Virions
Original language description
The Picornavirales include viruses that infect vertebrates, insects, and plants. It was believed that they pack only their genomic mRNA in the particles, thus, we envisaged these viruses as excellent model systems for studies of mRNA modifications. We used LC–MS to analyze digested RNA isolated from particles of the sacbrood and deformed wing iflaviruses as well as of the echovirus 18 and rhinovirus 2 picornaviruses. Whereas in the picornavirus RNAs we detected only N6-methyladenosine and 2’-O-methylated nucleosides, the iflavirus RNAs contained a wide range of methylated nucleosides, such as 1-methyladenosine (m1A) and 5-methylcytidine (m5C). Mapping of m1A and m5C through RNA sequencing of the SBV and DWV RNAs revealed the presence of tRNA molecules. Both modifications were detected only in tRNA. Further analysis revealed that tRNAs are present in form of 3’ and 5’ fragments and they are packed selectively. Moreover, these tRNAs are typically packed by other viruses.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Chembiochem
ISSN
1439-4227
e-ISSN
1439-7633
Volume of the periodical
23
Issue of the periodical within the volume
17
Country of publishing house
DE - GERMANY
Number of pages
9
Pages from-to
e202200281
UT code for WoS article
000828265300001
EID of the result in the Scopus database
2-s2.0-85134532067