Characterization of pyranose dehydrogenase from Agaricus meleagris and its application in the C-2 specific conversion of D-galactose
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F08%3A00313090" target="_blank" >RIV/61388971:_____/08:00313090 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Characterization of pyranose dehydrogenase from Agaricus meleagris and its application in the C-2 specific conversion of D-galactose
Original language description
Pyranose dehydrogenase of the mushroom Agaricus meleagris was purified from mycelial culture media using ion-exchange and hydrophobic interaction chromatography. The native enzyme is a monomeric polypeptide with a molecular mass of 66,547 Da, containing7% carbohydrate and covalently bound flavin adenine dinucleotide. The enzyme oxidized different aldopyranoses to the corresponding C-2 or C-2,3 (di)dehydro sugars. Preferred electron donors with the highest kcat/Km values were major sugar constituents ofcellulose and hemicellulose, namely D-glucose, D-galactose, L-arabinose, D-xylose and cellobiose
Czech name
Charakterizace pyranosadehydrogenasy z Agaricus meleagris a její využití k C-2 specifické konversi D-galaktosy
Czech description
Pyranosadehydrogenasa Agaricus meleagris byla purifikována z media myceliálních kultur této houby užitím ionexové a hydrofobně-interakční chromatografie. Nativní enzym byl charakterizován jako monomerní polypeptid o molekulární hmotnosti 66 547 Da, obsahující 7% sacharidu a kovalentně vázaný flavinadenindinukleotid. Oxidoval různé aldopyranosy na odpovídající C-2 či C-2,3 (di)dehydrocukry
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2008
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Biotechnology
ISSN
0168-1656
e-ISSN
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Volume of the periodical
133
Issue of the periodical within the volume
3
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
9
Pages from-to
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UT code for WoS article
000252632200009
EID of the result in the Scopus database
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