Three-Step Enzymatic Remodeling of Chitin into Bioactive Chitooligomers
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F24%3A00588023" target="_blank" >RIV/61388971:_____/24:00588023 - isvavai.cz</a>
Result on the web
<a href="https://pubs.acs.org/doi/10.1021/acs.jafc.4c03077" target="_blank" >https://pubs.acs.org/doi/10.1021/acs.jafc.4c03077</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.jafc.4c03077" target="_blank" >10.1021/acs.jafc.4c03077</a>
Alternative languages
Result language
angličtina
Original language name
Three-Step Enzymatic Remodeling of Chitin into Bioactive Chitooligomers
Original language description
Here we describe a complex enzymatic approach to the efficient transformation of abundant waste chitin, a byproduct of the food industry, into valuable chitooligomers with a degree of polymerization (DP) ranging from 6 to 11. This method involves a three-step process: initial hydrolysis of chitin using engineered variants of a novel fungal chitinase from Talaromyces flavus to generate low-DP chitooligomers, followed by an extension to the desired DP using the high-yielding Y445N variant of beta-N-acetylhexosaminidase from Aspergillus oryzae, achieving yields of up to 57%. Subsequently, enzymatic deacetylation of chitooligomers with DP 6 and 7 was accomplished using peptidoglycan deacetylase from Bacillus subtilis BsPdaC. The innovative enzymatic procedure demonstrates a sustainable and feasible route for converting waste chitin into unavailable bioactive chitooligomers potentially applicable as natural pesticides in ecological and sustainable agriculture.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Agricultural and Food Chemistry
ISSN
0021-8561
e-ISSN
1520-5118
Volume of the periodical
72
Issue of the periodical within the volume
28
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
15613-15623
UT code for WoS article
001272066200001
EID of the result in the Scopus database
2-s2.0-85198059256