Gluten-Free Diet Induces Small-Scale Changes Across Multiple T-Cell Subsets in NOD Mice

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F25%3A00639498" target="_blank" >RIV/61388971:_____/25:00639498 - isvavai.cz</a>
Alternative codes found
RIV/68378050:_____/25:00639498 RIV/60460709:41210/25:101879
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/10.1002/eji.202451559" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1002/eji.202451559</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/eji.202451559" target="_blank" >10.1002/eji.202451559</a>

Result language
angličtina
Original language name
Gluten-Free Diet Induces Small-Scale Changes Across Multiple T-Cell Subsets in NOD Mice
Original language description
Nonobese diabetic (NOD) mice are a widely used animal model to study mechanisms leading to autoimmune diabetes. A gluten-free diet reduces and delays the incidence of diabetes in NOD mice, but the underlying mechanisms remain largely unknown. In this study, we performed single-cell transcriptomic and flow cytometry analysis of T cells and innate lymphocytes in the spleen and pancreatic lymph nodes of NOD mice fed a gluten-free or standard diet. We observed that the gluten-free diet did not induce a substantial alteration in the abundance or phenotype of any lymphocyte subset that would directly explain its protective effect against diabetes. However, the gluten-free diet induced subtle changes in the differentiation of subsets with previously proposed protective roles in diabetes development, such as Tregs, activated gamma delta T cells, and NKT cells. Globally, the gluten-free diet paradoxically promoted activation and effector differentiation across multiple subpopulations and induced genes regulated by IL-2, IL-7, and IL-15. In contrast, the standard diet induced type I interferon-responsive genes. Overall, the gluten-free diet might prevent diabetes in NOD mice by inducing small-scale changes in multiple cell types rather than acting on a specific lymphocyte subset.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30102 - Immunology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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