Quantification of t-tubule area and protein distribution in rat cardiac ventricular myocytes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388998%3A_____%2F08%3A00087224" target="_blank" >RIV/61388998:_____/08:00087224 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14110/08:00024218

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Quantification of t-tubule area and protein distribution in rat cardiac ventricular myocytes

Original language description

The transverse (t-) tubules of cardiac ventricular myocytes are invaginations of the surface membrane that form a complex network within the cell. Many of the key proteins involved in excitation?contraction coupling appear to be located predominantly atthe t-tubule membrane. Despite their importance, the fraction of cell membrane within the ttubules remains unclear: measurement of cell capacitance following detubulation suggests 32%, whereas optical measurements suggest up to 65%. We have, therefore, investigated the factors that may account for this discrepancy. Calculation of the combinations of t-tubule radius, length and density that produce t-tubular membrane fractions of 32% or 56% suggest that the true fraction is at the upper end of this range. Assessment of detubulation using confocal and electron microscopy suggests that incomplete detubulation can account for some, but not all of the difference.

Czech name

Stanovení plochy t-tubulů a distribuce transportních bílkovin u srdečních komorových buněk potkana

Czech description

Práce prezentuje využití matematického modelu pro určení frakční plochy tubulární membrány a pro odhad distribuce transportních bílkovin mezi periferní a tubulární membránou u srdečních komorových buněk laboratorního potkana.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Progress in Biophysics & Molecular Biology

ISSN

0079-6107

e-ISSN

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Volume of the periodical

96

Issue of the periodical within the volume

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Country of publishing house

GB - UNITED KINGDOM

Number of pages

14

Pages from-to

244-257

UT code for WoS article

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EID of the result in the Scopus database

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