Magnetic poly(glycidyl methacrylate) microspheres for protein capture
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F14%3A00430063" target="_blank" >RIV/61389013:_____/14:00430063 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.nbt.2014.06.004" target="_blank" >http://dx.doi.org/10.1016/j.nbt.2014.06.004</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.nbt.2014.06.004" target="_blank" >10.1016/j.nbt.2014.06.004</a>
Alternative languages
Result language
angličtina
Original language name
Magnetic poly(glycidyl methacrylate) microspheres for protein capture
Original language description
The efficient isolation and concentration of protein antigens from complex biological samples is a critical step in several analytical methods, such as mass spectrometry, flow cytometry and immunochemistry. These techniques take advantage of magnetic microspheres as immunosorbents. The focus of this study was on the development of new superparamagnetic polymer microspheres for the specific isolation of the tumor suppressor protein p53. Monodisperse macroporous poly(glycidyl methacrylate) (PGMA) microspheres measuring approximately 5 ?m and containing carboxyl groups were prepared by multistep swelling polymerization of glycidyl methacrylate (GMA), 2-[(methoxycarbonyl)methoxy]ethyl methacrylate (MCMEMA) and ethylene dimethylacrylate (EDMA) as a crosslinker in the presence of cyclohexyl acetate as a porogen. To render the microspheres magnetic, iron oxide was precipitated within their pores; the Fe content in the particles received 18 wt%. Nonspecific interactions between the magnetic pa
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CD - Macromolecular chemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
New Biotechnology
ISSN
1871-6784
e-ISSN
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Volume of the periodical
31
Issue of the periodical within the volume
5
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
10
Pages from-to
482-491
UT code for WoS article
000341314700012
EID of the result in the Scopus database
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