Use of specific polysaccharide-immobilized monodisperse poly(glycidyl methacrylate) core-silica shell microspheres for affinity purification of lectins

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F15%3A00443497" target="_blank" >RIV/61389013:_____/15:00443497 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1002/bmc.3360" target="_blank" >http://dx.doi.org/10.1002/bmc.3360</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/bmc.3360" target="_blank" >10.1002/bmc.3360</a>

Result language

angličtina

Original language name

Use of specific polysaccharide-immobilized monodisperse poly(glycidyl methacrylate) core-silica shell microspheres for affinity purification of lectins

Original language description

Immobilization of polysaccharides (yeast mannan and gum arabic) on the macroporous poly(glycidyl methacrylate) monodisperse microspheres coated with silica (SiO2)-containing amino groups on the surface was used to prepare affinity sorbents for lectin purification. The efficiency of isolating mannose specific Pisum sativum lectin was demonstrated on sorbent with immobilized yeast mannan and that of galactose specific Glycine hispida lectin on sorbent with immobilized gum arabic. The microspheres with immobilized polysaccharides can be used for selecting an affinity sorbent for purification of other mannose- and galactose-specific lectins. In contrast to yeast mannan, the gum arabic immobilized on the microspheres possesses much narrower specificity andis suitable for purification of only those galactose specific lectins which interact well with l-rhamnose or l-arabinose. The synthesized macroporous particles are capable of immobilizing 50?mg of polysaccharide per 1?g of the matrix, whi

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CD - Macromolecular chemistry

OECD FORD branch

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Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biomedical Chromatography

ISSN

0269-3879

e-ISSN

—

Volume of the periodical

29

Issue of the periodical within the volume

5

Country of publishing house

GB - UNITED KINGDOM

Number of pages

5

Pages from-to

783-787

UT code for WoS article

000353880000021

EID of the result in the Scopus database

2-s2.0-84927773173