NaYF4-based upconverting nanoparticles with optimized phosphonate coatings for chemical stability and viability of human endothelial cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F22%3A00550727" target="_blank" >RIV/61389013:_____/22:00550727 - isvavai.cz</a>

Result on the web

<a href="https://iopscience.iop.org/article/10.1088/2050-6120/ac41ba" target="_blank" >https://iopscience.iop.org/article/10.1088/2050-6120/ac41ba</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1088/2050-6120/ac41ba" target="_blank" >10.1088/2050-6120/ac41ba</a>

Result language

angličtina

Original language name

NaYF4-based upconverting nanoparticles with optimized phosphonate coatings for chemical stability and viability of human endothelial cells

Original language description

The increasing interest in upconverting nanoparticles (UCNPs) in biodiagnostics and therapy fuels the development of biocompatible UCNPs platforms. UCNPs are typically nanocrystallites of rare-earth fluorides codoped with Yb3+ and Er3+ or Tm3+. The most studied UCNPs are based on NaYF4 but are not chemically stable in water. They dissolve significantly in the presence of phosphates. To prevent any adverse effects on the UCNPs induced by cellular phosphates, the surfaces of UCNPs must be made chemically inert and stable by suitable coatings. We studied the effect of various phosphonate coatings on chemical stability and in vitro cytotoxicity of the Yb3+,Er3+-codoped NaYF4 UCNPs in human endothelial cells obtained from cellular line Ea.hy926. Cell viability of endothelial cells was determined using the resazurin-based assay after the short-term (15 min), and long-term (24 h and 48 h) incubations with UCNPs dispersed in cell-culture medium. The coatings were obtained from tertaphosphonic acid (EDTMP), sodium alendronate and poly(ethylene glycol)-neridronate. Regardless of the coating conditions, 1 − 2 nm-thick amorphous surface layers were observed on the UCNPs with transmission electron microscopy. The upconversion fluorescence was measured in the dispersions of all UCNPs. Surafce quenching in aqueous suspensions of the UCNPs was reduced by the coatings. The dissolution degree of the UCNPs was determined from the concentration of dissolved fluoride measured with ion-selective electrode after the ageing of UCNPs in water, physiological buffer (i.e., phosphate-buffered saline—PBS) and cell-culture medium. The phosphonate coatings prepared at 80 °C significantly suppressed the dissolution of UCNPs in PBS while only minor dissolution of bare and coated UCNPs was measured in water and cell-culture medium. The viability of human endothelial cells was significantly reduced when incubated with UCNPs, but it increased with the improved chemical stability of UCNPs by the phosphonate coatings with negligible cytotoxicity when coated with EDTMP at 80 °C.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10404 - Polymer science

Project

<a href="/en/project/GA21-04420S" target="_blank" >GA21-04420S: Protective coatings against dissolution/disintegration of upconverting lanthanide-based nanoparticles in biological media</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Methods and Applications in Fluorescence

ISSN

2050-6120

e-ISSN

2050-6120

Volume of the periodical

10

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

10

Pages from-to

014001

UT code for WoS article

000733008900001

EID of the result in the Scopus database

2-s2.0-85122904275