Usefulness of embryogenic cell suspension cultures for the induction and selection of mutations in Musa spp

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F04%3A00107481" target="_blank" >RIV/61389030:_____/04:00107481 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Usefulness of embryogenic cell suspension cultures for the induction and selection of mutations in Musa spp

Original language description

In bananas shoot-tip cultures are traditionally used for mutagenesis. The main problem with this type of culture is the presence of chimerism. In contrast, embryogenic cell suspensions allow the handling of large populations under controlled conditions and simultaneously avoid chimerism if embryos have a single-cell origin. In this study, we verified the unicellular origin of somatic embryos by treating embryogenic cell suspensions (ECS) with colchicine and determining the ploidy of regenerated plants by flow cytometry. We have found that none of the plants regenerated from colchicine-treated ECS was mixoploid (chimeric). To optimise a protocol for the irradiation of ECS, several experiments were performed. Flow cytometric analysis of cell-cycle kinetics proved to be more informative than growth curves for the determination of the optimal timing of irradiation. The optimal irradiation dose was determined using three different parameters. Among them, the fresh weight gain and the reg...

Czech name

Použití suspenzí buněčných kultur pro indukci a selekci mutantů u rodu Musa

Czech description

Banánových kořenových špiček je tradičně používáno při mutagenezi. Hlavní nevýhodou těchto kultur je ale chimerismus. Tato studie popisuje použití metody vytváření somatických embryí z embryonálních buněčných kultur ovlivněných kolchicinem. Po analýze ploidie průtokovou cytometrí nebyl zaznamenán chimerismus.

Type

C - Chapter in a specialist book

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/IAA6038204" target="_blank" >IAA6038204: Construction of banana bacterial artificial chromosome (BAC) library for physical genome mapping</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Book/collection name

Banana Improvement: Cellular, Molecular Biology, and Induced Mutations

ISBN

1-57808-340-0

Number of pages of the result

11

Pages from-to

33-43

Number of pages of the book

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Publisher name

Science Publishers, Inc

Place of publication

Enfield

UT code for WoS chapter

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