Detecting ploidy level instability of banana embryogenic cell suspension cultures by flow cytometry

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F04%3A00107484" target="_blank" >RIV/61389030:_____/04:00107484 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Detecting ploidy level instability of banana embryogenic cell suspension cultures by flow cytometry

Original language description

During micropropagation of bananas and plantains, somaclonal variation can occur in regenerated plantlets. This variation may interfere with the use of these cultures for physical or chemical mutagenesis and/or genetic transformation. Although the causesof genetic instability are poorly understood, chromosome instability is believed to be one of the most common causes of tissue culture-induced variation. Using flow cytometry, variation in chromosome number could be detected in embryogenic cell suspensions and in plants regenerated from them. Results obtained by flow cytometry were verified by chromosome counting in meristem root-tip cells. Abnormalities in DNA content such as polyploidy and aneuploidy were detected at the cell suspension level. For the first time, a hypotriploid banana embryogenic cell line with 2n = 28 (i.e. with loss of five chromosomes) was reported. Factors affecting the genetic stability of embryogenic cell suspensions are discussed.

Czech name

Detekce nestability ploidie embryogenních buněk banánovníku pomocí průtokové cytometrie

Czech description

V průběhu mikropropagace banánovníků se objevuje mezi regenerovanými rostlinami somaklonální variabilita. Varabilita v počtu chromozómů může být u regenerovaných rostlin detekována pomocí průtokové cytometrie. Výsledky dosažené pomocí průtokové cytometrie byly potvrzeny počítáním chromozómů v kořenovém meristému. Poprvé byl detekován hypotriploidní jedinec (2n = 28). Dále jsou diskutovány faktory ovlivňující genetickou stabilitu embriogenních kultur.

Type

C - Chapter in a specialist book

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/IAA6038204" target="_blank" >IAA6038204: Construction of banana bacterial artificial chromosome (BAC) library for physical genome mapping</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Book/collection name

Banana Improvement: Cellular, Molecular Biology, and Induced Mutations

ISBN

1-57808-340-0

Number of pages of the result

11

Pages from-to

251-261

Number of pages of the book

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Publisher name

Science Publishers, Inc

Place of publication

Enfield

UT code for WoS chapter

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