Dependence of Chlorophyll Fluorescence Quenching on the Lipid-to-Protein Ratio in Reconstituted Light-Harvesting Complex II Membranes Containing Lipid Labels

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F19%3AA2001YX4" target="_blank" >RIV/61988987:17310/19:A2001YX4 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0301010418314186" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0301010418314186</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chemphys.2019.03.012" target="_blank" >10.1016/j.chemphys.2019.03.012</a>

Result language
angličtina
Original language name
Dependence of Chlorophyll Fluorescence Quenching on the Lipid-to-Protein Ratio in Reconstituted Light-Harvesting Complex II Membranes Containing Lipid Labels
Original language description
The quenching of chlorophyll-a fluorescence was investigated in plant light-harvesting complex II (LHCII) embedded in reconstituted membranes containing thylakoid lipids and a lipid label. The proteoliposomes were further separated by density and the protein and lipid contents of the fractions were quantified spectrophotometrically, allowing tighter control over the L/P ratios in a wide range of values. Using time-resolved fluorescence, we found a strong correlation between the fluorescence quenching and the L/P ratio, in line with other studies reporting progressive quenching at low L/P ratios, presumably triggered by self-clustering in the membrane.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10610 - Biophysics

Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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