Nucleotides in Erythrocytes - Incorporation Study

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F05%3A10213690" target="_blank" >RIV/61989592:15110/05:10213690 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Nucleotides in Erythrocytes - Incorporation Study

Original language description

Objective: Analysis of nucleotides for diagnosing inborn errors of metabolism. Transport studies of purine ribosides into erythrocytes. Material and Methods: Erythrocytes were incubated with purine ribosides. Ribosides were transported through erythrocyte membrane and converted into ribotides. Nucleotides were separated and identified by capillary electrophoretic method using background electrolyte 40 mmol/L citric acid ? 0.8 mmol/L cetyltrimethylammonium bromide-gama-aminobutyric acid, pH 4.4. Results:We found accumulated deoxynucleotides and nucleotides in erythrocyte extracts, which mimics metabolic situation of enzyme defects of purine metabolism. Under optimal electrophoretic conditions all compounds of interest were separated. Conclusion: The method can be successfully used to analyze intracellular nucleotides and diagnose inherited disorders of nucleotide metabolism.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/NR7796" target="_blank" >NR7796: PURINE DE NOVO SYNTHESIS DEFECTS - METABOLIC STUDY</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Klinická biochemie a metabolismus

ISSN

1210-7921

e-ISSN

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Volume of the periodical

13

Issue of the periodical within the volume

4

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

2

Pages from-to

182-183

UT code for WoS article

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EID of the result in the Scopus database

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