Analysis of aminoimidazole ribosides by capillary electrophoresis-diagnosing defects in second part of purine biosynthetic pathway.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F07%3A00004487" target="_blank" >RIV/61989592:15110/07:00004487 - isvavai.cz</a>
Alternative codes found
RIV/00098892:_____/07:#0000055 RIV/61989592:15110/07:00003926
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Analysis of aminoimidazole ribosides by capillary electrophoresis-diagnosing defects in second part of purine biosynthetic pathway.
Original language description
Only three inherited metabolic defects have been identified in purine de novo synthesis (PDNS). We present here CE methods for diagnosing defects in the second half of PDNS (from sixth to tenth enzymatic conversion) based on analysis of aminoimidazole ribosides - dephosphorylated intermediates - in urine. METHODS: Assays were performed in an uncoated fused-silica capillary using two electrophoretic separation systems: 60 mmol/l borate - 2-amino-2-methyl-1-propanol-80 mmol/l sodium dodecylsulfate (pH 9.6) and 200 mmol/l phosphate - sodium (pH 1.8). RESULTS: The reported conditions allowed separation of all metabolites from major urinary constituents with analysis time less than 10 min and separation efficiency of 220 and 350 thousands theoretical platesper meter for borate and phosphate system, respectively. The intra- and interday imprecisions were less than 4.4% and 9.9% CV. Potential usefulness of the methods was demonstrated on samples from a patient with adenylosuccinate lyase def
Czech name
Analýza aminoimidazolových ribozidů kapilární elektroforézou-diagnostika defektů v druhé části purinové biosyntézy.
Czech description
Dosud byly identifikovány v purinové de novo syntéze pouze tři dědičné metabolické defekty (PDNS). Zde se pojednává o CE metodách určených pro diagnostiku defektů v druhé polovině PDNS (od šesté do desáté enzymatické konverze) založenou na analýze imidazolových ribozidů.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FD - Oncology and haematology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2007
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Clinica Chimica Acta
ISSN
0009-8981
e-ISSN
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Volume of the periodical
376
Issue of the periodical within the volume
1-2
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
6
Pages from-to
184-189
UT code for WoS article
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EID of the result in the Scopus database
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