HDAC inhibitors sodium butyrate and sodium valproate do not affect human NCOR1 and NCOR2 gene expression in HL-60 cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F11%3A10223831" target="_blank" >RIV/61989592:15110/11:10223831 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

HDAC inhibitors sodium butyrate and sodium valproate do not affect human NCOR1 and NCOR2 gene expression in HL-60 cells

Original language description

We examined whether histone deacetylase (HDAC) inhibitors butyrate and valproate alter expression of NCOR1 and NCOR2 genes coding for N-CoR and SMRT, respectively. Leukemic HL-60 cells were treated for 24 h with 0.5 and 1 mM butyrate, 1-3 mM valproate, 1?M retinoic acid (RA) or cotreated with 1 ?M RA and 0.5 mM butyrate. Histone acetylation was analysed by western blotting. Levels of NCOR1 and NCOR2 mRNA were determined by real-time PCR. Expression of NCF2 gene coding for p67phox was evaluated as myeloid differentiation marker. Butyrate and valproate increased acetylation of histones H3 and H4. They also increased NCF2 mRNA without affecting NCOR1 or NCOR2 mRNA levels. Similarly, RA alone or in combination with butyrate induced NCF2 gene expression without affecting NCOR1 or NCOR2 mRNA levels. We conclude that HDAC inhibitors do not alter expression of NCOR1 or NCOR2 genes and that the onset of myeloid differentiation is not accompanied by induction or repression of these genes in HL-

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/ED0030%2F01%2F01" target="_blank" >ED0030/01/01: Biomedicine for regional development and human resources</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biomedical Papers-Olomouc

ISSN

1213-8118

e-ISSN

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Volume of the periodical

155

Issue of the periodical within the volume

3

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

4

Pages from-to

259-262

UT code for WoS article

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EID of the result in the Scopus database

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