Potential of High-Performance Liquid Chromatography for Distribution Coefficient Determination of 3-Hydroxyquinolin-4(1H)-one Derivatives

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F16%3A33160887" target="_blank" >RIV/61989592:15110/16:33160887 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15310/16:33160887

Result on the web

<a href="http://link.springer.com/article/10.1007/s10337-016-3129-6/fulltext.html" target="_blank" >http://link.springer.com/article/10.1007/s10337-016-3129-6/fulltext.html</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10337-016-3129-6" target="_blank" >10.1007/s10337-016-3129-6</a>

Result language

angličtina

Original language name

Potential of High-Performance Liquid Chromatography for Distribution Coefficient Determination of 3-Hydroxyquinolin-4(1H)-one Derivatives

Original language description

The potential of reversed-phase HPLC for the determination of distribution coefficient D 7.4 of selected 3-hydroxyquinolin-4(1H)-ones (3HQs) as compounds with significant biological activity was studied. Various stationary phases with C18 as well as hexyl-phenyl modification reflect current trends in RP-HPLC development such as higher sorbent silanophilicity, core-shell technology, hybrid and/or charged surface particles. Because of significant peak tailing of 3HQs at physiological pH on reversedphase sorbents the separations at pH 3 were performed as well. Surprisingly, the pH change did not affect significantly the partition coefficients of 3HQs. Very affordable and common standards such as anisole, acetophenone, benzyl alcohol, brombenzene, ethylbenzoate and trichlore- thylene were applied in the described methodology. The best linearity (R 2 0.9895) of the correlation between log P and log kw for standards was obtained for hexyl-phenyl sorbent, but this stationary phase was shown to be unsuit- able for HPLC separation of 3HQs. The highest linearity (R2 0.9499) of the relationship between log D7.4 determined by the classic shake-flask method and log D determined by means of HPLC for 3HQs was attained with Cortecs C18+ column at pH 7.4. The described methodology with Cortecs C18+ as stationary phase offers fast and accurate estimation of log D 7.4 of the tested 3HQs. In an effort to increase the throughput of the HPLC method for log D7.4

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/LO1304" target="_blank" >LO1304: Support of suistainability of the Institute of Molecular and Translational Medicine</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chromatographia

ISSN

0009-5893

e-ISSN

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Volume of the periodical

79

Issue of the periodical within the volume

17

Country of publishing house

DE - GERMANY

Number of pages

11

Pages from-to

1153-1163

UT code for WoS article

000382010000010

EID of the result in the Scopus database

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