Testing the adherence of commensal and pathogenic bacteria to monolayers of human intestinal epithelial cells as means for selection of bacteria-vectored recombinant vaccines

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F18%3A73591313" target="_blank" >RIV/61989592:15110/18:73591313 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Testing the adherence of commensal and pathogenic bacteria to monolayers of human intestinal epithelial cells as means for selection of bacteria-vectored recombinant vaccines

Original language description

Mucosal vaccination induces antigen-specific humoral and cell-mediated immune responses in the systemic and mucosal compartments. Most frequently used oral administration of vaccine induces the production of antigen-specific secretory IgA (S-IgA) antibodies in secretion of the gastrointestinal tract, as well as in saliva, milk, and tears. Because S-IgA contributes to the natural barrier function against the entry of pathogenic bacteria through mucosal surfaces, we examined the mechanisms involved in the interaction of antibodies with attenuated bacteria and epithelial cells.In our studies we focused on interactions of humanintestinal epithelial cell lines Caco-2 and Hiec-1 with pathogenic and non-pathogenic strains of Escherichia coli and S-IgA from humanmilk. As reported earlier, epithelial cells bind E. colithrough the glycoprotein receptors expressed on cell surfaces which recognizebacterial pili. These interactions can be efficiently inhibited by S-IgA which binds E. colithrough the specific antibody activity or S-IgA-associated glycans. In our studies we addressed the questions concerning the ability of pathogenic bacteria to overcome the protective activity of S-IgA and comparedthe extent of binding of pathogenic and non-pathogenic bacteria to glycoprotein receptors.The pathogenic E. colistrain O55 and non-pathogenic strains O86 and S16 were incubated with S-IgA and subsequently with the epithelial cell lines. The numbers of adhered bacteria were determined after extensive washing. To provide evidence for the S-IgA glycan-mediated inhibition of bacterial adherence to epithelialcells, S-IgA was incubated with PNGase F enzyme which cleaves Nlinked glycans. This treatment resulted in a significant increase of bacterial adherence to epithelial cells.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

30102 - Immunology

Project

<a href="/en/project/EF16_025%2F0007397" target="_blank" >EF16_025/0007397: Recombinant Biotechnology and Immunotherapy Centre</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

11th International Conference Drug Delivery Systems Nanotechnology for Healthcare: Progress in Recombinant Vaccines, Molecular Adjuvants, Modern Drug Delivery Systems and Cell Therapy

ISBN

978-80-907074-6-7

ISSN

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e-ISSN

neuvedeno

Number of pages

12

Pages from-to

47-58

Publisher name

nakladatelství Machovský

Place of publication

Olomouc

Event location

Telč

Event date

Jun 5, 2018

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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