Human keratinocyte cell line as a suitable alternative model for in vitro phototoxicity testing

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F19%3A73594099" target="_blank" >RIV/61989592:15110/19:73594099 - isvavai.cz</a>

Result on the web

<a href="http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0365-05962019000100021&lng=en&nrm=iso&tlng=en" target="_blank" >http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0365-05962019000100021&lng=en&nrm=iso&tlng=en</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1590/abd1806-4841.20197620" target="_blank" >10.1590/abd1806-4841.20197620</a>

Result language

angličtina

Original language name

Human keratinocyte cell line as a suitable alternative model for in vitro phototoxicity testing

Original language description

The 3T3 Neutral Red Uptake Phototoxicity Test (3T3NRUPT) is the only validated in vitro assay for compound phototoxicity testing that replaces the use of animals for testing cosmetics, which is banned in Europe. 3T3NRUPT uses the mouse fibroblast cell line Balb/c 3T3 (Balb/c). When the test was introduced in our studies, we dealt with a problem with Balb/c detachment from culture plates during the washing steps and following incubation in phosphate buffered saline (PBS). In order to improve the model system, we compared the attachment of Balb/c on various plate surfaces. We also tested the effect of different buffered saline solutions. In parallel, experiments were performed on a human keratinocyte cell line (HaCaT). The response of both cell lines to chlorpromazine, a recommended phototoxic compound in the validated 3T3NRUPT protocol, was also compared. Results showed a dramatic reduction in the number of Balb/c after the washing steps and subsequent incubation in buffered saline solution. In contrast, HaCaT cells were minimally affected. No beneficial effect on Balb/c number was found for any of the tested surfaces and/or buffered saline solutions. In conclusion, HaCaT cells are minimally affected during the phototoxicity assay and seems to be a suitable alternative alongside Balb/c especially for testing compounds intended to be applied topically.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/GA15-10897S" target="_blank" >GA15-10897S: Modulation of human skin cell responses to UVA radiation by silymarin components</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

ANAIS BRASILEIROS DE DERMATOLOGIA

ISSN

0365-0596

e-ISSN

—

Volume of the periodical

94

Issue of the periodical within the volume

1

Country of publishing house

BR - BRAZIL

Number of pages

2

Pages from-to

105-106

UT code for WoS article

000457571600021

EID of the result in the Scopus database

2-s2.0-85061159042