Analysis of buserelin in urine by online combination of capillary zone electrophoresis with electrospray mass spectrometry

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F10%3A10215687" target="_blank" >RIV/61989592:15310/10:10215687 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Analysis of buserelin in urine by online combination of capillary zone electrophoresis with electrospray mass spectrometry

Original language description

A fast and precise analysis of the synthetic peptide buserelin in urine using CZE-ESI-MS method has been demonstrated. Formic acid at 50 mmol/L concentration served as backgroud electrolyte in CZE stage and it is compatible with MS detection in positiveionization mode. Two injection modes were tested, i.e. pressure (50 mbar for 5 s) and electrokinetic injection (5 kV for 5 s), of which electrokinetic injection provided better calibration parameters. Buserelin LODs were 0 47 mu g/mL in water and 0 63 mug/mL in ten times diluted urine samples using pressure injection, while they were 0.32 mu g/mL in water and 0.34 mu g/mL in ten times diluted urine samples using electrokinetic injection Repeatability of buserelin migration times was below 6% (pressureinjection mode) and 1% (electrokinetic injection mode).

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

—

Project

—

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electrophoresis

ISSN

0173-0835

e-ISSN

—

Volume of the periodical

31

Issue of the periodical within the volume

7

Country of publishing house

DE - GERMANY

Number of pages

7

Pages from-to

—

UT code for WoS article

000276811000015

EID of the result in the Scopus database

—