Enzyme based amperometric biosensor for adenine determination
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F13%3A33147808" target="_blank" >RIV/61989592:15310/13:33147808 - isvavai.cz</a>
Result on the web
<a href="http://onlinelibrary.wiley.com/doi/10.1002/elan.201200387/abstract;jsessionid=2C1433B959A1A394B4B81EE2A1CD99CD.f03t01" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/elan.201200387/abstract;jsessionid=2C1433B959A1A394B4B81EE2A1CD99CD.f03t01</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elan.201200387" target="_blank" >10.1002/elan.201200387</a>
Alternative languages
Result language
angličtina
Original language name
Enzyme based amperometric biosensor for adenine determination
Original language description
An enzyme-based amperometric biosensor was developed for the rapid determination of adenine in biological and food samples. Recombinant adenine deaminase (EC 3.5.4.2) from Schizosaccharomyces pombe was co-immobilized on graphite electrode with xanthine oxidase (EC 1.17.3.2), peroxidase (EC 1.11.1.7) and an Os mediator. Optimal working conditions were pH 7.0 and 150 mV vs. Ag/AgCl. The novel method was characterized by a detection limit of 1 m M and a linear measuring range of 0.005-0.1 mM. Different beer and tea samples were analyzed by the newly elaborated adenine biosensor. The results obtained correlate with the adenine content measured by the reference HPLC method.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
<a href="/en/project/MEB040906" target="_blank" >MEB040906: Application of enzymes for accelerated assessment of food safety</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electroanalysis
ISSN
1040-0397
e-ISSN
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Volume of the periodical
25
Issue of the periodical within the volume
1 - SI
Country of publishing house
DE - GERMANY
Number of pages
6
Pages from-to
237-242
UT code for WoS article
000313807600030
EID of the result in the Scopus database
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