Effects of fish oil diet on M1 and M2 monocyte derived macrophages polarization
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F15%3A43906869" target="_blank" >RIV/62156489:43210/15:43906869 - isvavai.cz</a>
Alternative codes found
RIV/00027162:_____/15:#0001237
Result on the web
<a href="https://mnet.mendelu.cz/mendelnet2015/mnet_2015_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2015/mnet_2015_full.pdf</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Effects of fish oil diet on M1 and M2 monocyte derived macrophages polarization
Original language description
The aim of this study was demonstrated effect of fish oil diet on M1 and M2 polarization of macrophages. Six piglets were fed with standard diet supplemented with 2.5% fish oil containing eicosapentaenoic acid (EPA) and six piglets were fed with addition2.5% palm oil as control group. We obtained mononuclear fraction of white blood cells from peripheral blood and we subsequently obtained CD14+ monocytes by magnetic separation. After 7 days of cultivation we obtained monocyte-derived macrophages (MDMF).It was measured genes expressions of pro-inflammatory soluble factors (IL-1?, TNF-? and MMP12) and anti-inflammatory (IL-10 and TIMP1) for detection of M1 or M2 polarization of MDMF. In the diet with fish oil, it showed a statistically significant increase in gene expression of MMP12 (P<0.01). It was measured genes expressions after stimulation of lipopolysaccharide (LPS). In case of both diet (fish oil and palm oil) IL-1 gene expression was increased in contrast to HPRT-1 (housekeeping
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
GJ - Diseases and animal vermin, veterinary medicine
OECD FORD branch
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Result continuities
Project
<a href="/en/project/LO1218" target="_blank" >LO1218: Healthy animal as a source of wholesome food</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
MendelNet 2015: Proceedings of International PhD Students Conference
ISBN
978-80-7509-363-9
ISSN
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e-ISSN
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Number of pages
5
Pages from-to
384-388
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Nov 11, 2015
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000366466100070