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Comparison of FT-NIR spectroscopy and ELISA for detection of adulteration of goat cheeses with cow's milk

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F16%3A43907972" target="_blank" >RIV/62156489:43210/16:43907972 - isvavai.cz</a>

  • Alternative codes found

    RIV/70883521:28110/16:43874511

  • Result on the web

    <a href="https://doi.org/10.5740/jaoacint.15-0190" target="_blank" >https://doi.org/10.5740/jaoacint.15-0190</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.5740/jaoacint.15-0190" target="_blank" >10.5740/jaoacint.15-0190</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Comparison of FT-NIR spectroscopy and ELISA for detection of adulteration of goat cheeses with cow's milk

  • Original language description

    This study investigated the ability of two methods to detect adulteration of goat cheeses via the addition of cow's milk, with a negligible effect on the raw materials. Cheeses were produced from a mixture of goat's and cow's milk and were then analyzed by Fourier transform near-IR (FT-NIR) spectroscopy and competitive ELISA. The cheese spectra were scanned in the spectroscope in reflectance mode on an integrating sphere at 80 scans and a resolution of 4 cmMINUS SIGN 1. The spectra were evaluated via discriminant analysis, and a calibration was created via a partial least-squares algorithm to quantify the cow's milk admixture. A correlation coefficient of R = 0.999 was reached with a standard error of calibration of 0.0407. The results were statistically processed to a median value via a t-test. Adulteration detection by the ELISA method was performed using a commercial Milk Fraud/Bovine ELISA kit. It was found that the FT-NIR spectroscopy method is capable of detecting an admixture of cow's milk in goat cheese as small as 1%. The ELISA method did not return satisfactory results for the detection of adulteration with cow's milk.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    GM - Food industry

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/QJ1230044" target="_blank" >QJ1230044: Determination of parameters for legislative assessing of the quality and safety of raw milk from cows, sheep and goats</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2016

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of AOAC International

  • ISSN

    1060-3271

  • e-ISSN

  • Volume of the periodical

    99

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    7

  • Pages from-to

    180-186

  • UT code for WoS article

    000371953400022

  • EID of the result in the Scopus database

    2-s2.0-84962374828