Morphological changes of monocytes during dendritic cells development

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F19%3A43917114" target="_blank" >RIV/62156489:43210/19:43917114 - isvavai.cz</a>
Result on the web
<a href="https://mnet.mendelu.cz/mendelnet2019/mnet_2019_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2019/mnet_2019_full.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Morphological changes of monocytes during dendritic cells development
Original language description
The aim of this work was the isolation of monocytes from bovine blood with the use of immunomagnetic beads coated with the monoclonal antibody, and magnetic separation and morphology determination of DCs generated from the monocytes. The experiment was performed on a group of healthy cows. Peripheral blood mononuclear cells were isolated from 100 ml of whole blood. Samples were incubated and centrifuged to achieve the desired concentration for the experiment. The isolated monocytes were cultured in the concentration of 1 x 106 cells/mL in standard culture flasks in RPMI 1640 medium at 37 oC in a humidified 5% CO2 atmosphere in the presence of granulocytemacrophage colony-stimulating factor (GM-CSF) and recombinant bovine interleukin-4 (IL-4). Cells were cultivated for 3 days. Cells transformed into DCs during this time. Morphology of DCs was observed in the light microscope. During 3 days of cultivation, there were found out morphological changes in the cell membrane of the cells. On the cell membrane, there were developed protrusions and during the time of incubation, there were developed typically elongated pseudopodia.
Czech name
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Czech description
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Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
MendelNet 2019: Proceedings of International PhD Students Conference
ISBN
978-80-7509-688-3
ISSN
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e-ISSN
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Number of pages
4
Pages from-to
470-473
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Nov 6, 2019
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000576735500084

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