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The determination of deoxynivalenol and zearalenone in barely from Brazil and malted barley

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F19%3A43917133" target="_blank" >RIV/62156489:43210/19:43917133 - isvavai.cz</a>

  • Result on the web

    <a href="https://mnet.mendelu.cz/mendelnet2019/mnet_2019_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2019/mnet_2019_full.pdf</a>

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    The determination of deoxynivalenol and zearalenone in barely from Brazil and malted barley

  • Original language description

    The present study describes the determination of mycotoxins deoxynivalenol (DON) and zearalenone (ZEA) in barley from Brazil and in malted barley. The quality of the raw materials involved in the malting process plays a decisive role in the creation of a safe beer product. Immunoaffinity columns for extraction and clean-up, and liquid chromatography coupled with mass spectrometry (LC-MS) are the most commonly used method for determining the presence of mycotoxins. A total of 24 samples were analysed for DON and ZEA. The average concentration of DON and ZEA was 2859.5 μg/kg and 727.9 μg/kg for barley and 1057.3 μg/kg and 275.1 μg/kg for malt, respectively. The content of mycotoxin decreases by about 63% during the malting process. The validation parameters of an analytical method such as linearity, limit of detection (LOD) and limit of quantification (LOQ), and recovery were tested. The calibration curves were linear at least in the range of 10-1000 ng/mL, LOD and LOQ ranging 2.8-3.5 μg/kg and 9.2-11.6 μg/kg, respectively. Recovery for DON and ZEA ranged 87.6%-92.9% with a RSD range of 2.9%-9.2% and 89.6%-105.7% with a RSD range of 0.4%-5.3%, respectively.

  • Czech name

  • Czech description

Classification

  • Type

    D - Article in proceedings

  • CEP classification

  • OECD FORD branch

    40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Result continuities

  • Project

    <a href="/en/project/TE02000177" target="_blank" >TE02000177: Centre for Innovative Use and Strengthening of Competitiveness of Czech Brewery Raw Materials and Products</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2019

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    MendelNet 2019: Proceedings of International PhD Students Conference

  • ISBN

    978-80-7509-688-3

  • ISSN

  • e-ISSN

  • Number of pages

    5

  • Pages from-to

    596-600

  • Publisher name

    Mendelova univerzita v Brně

  • Place of publication

    Brno

  • Event location

    Brno

  • Event date

    Nov 6, 2019

  • Type of event by nationality

    WRD - Celosvětová akce

  • UT code for WoS article

    000576735500107