Development of RT-PCR method for detecting GCLV by specific primers
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F16%3A43908792" target="_blank" >RIV/62156489:43510/16:43908792 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.17660/ActaHortic.2016.1110.4" target="_blank" >http://dx.doi.org/10.17660/ActaHortic.2016.1110.4</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17660/ActaHortic.2016.1110.4" target="_blank" >10.17660/ActaHortic.2016.1110.4</a>
Alternative languages
Result language
angličtina
Original language name
Development of RT-PCR method for detecting GCLV by specific primers
Original language description
The method was developed for the detection and resolution of the Garlic common latent virus that causes a serious disease of economically important plants of Allium species, in the Potyvirus presence particularly. GCLV was detected in garlic from Asia, Europe, South America and also from North America. GCLV together with SLV were the most widespread in the collection, as they occurred in 82 and 83% of the accessions in the Czech Republic as was described earlier. GCLV belong to ssRNA positive-strand viruses and is encoded of a single molecule of linear ssRNA of ~8.6 kb, RT-PCR was developed because it is a suitable detection method for RNA viruses. For the development of the RT-PCR method for detecting GCLV two primers were designed which amplify part of genome which encode gene NABP (nucleic acid binding proteins family). These primers amplify a product of approximately 782 base pairs. The product can be easily visualized on agarose gel electrophoresis. RT-PCR conditions were fully optimized for the detection GCLV in infected plants. This is a report on the development of a method for quick and easy, sensitive and economically reasonable virus detection GCLV
Czech name
—
Czech description
—
Classification
Type
D - Article in proceedings
CEP classification
GF - Diseases, pests, weeds and plant protection
OECD FORD branch
—
Result continuities
Project
<a href="/en/project/EE2.4.31.0089" target="_blank" >EE2.4.31.0089: Cooperation Network for Horticulture and Landscape Architecture</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Acta Horticulturae 1110
ISBN
978-94-6261-104-7
ISSN
0567-7572
e-ISSN
—
Number of pages
6
Pages from-to
21-26
Publisher name
International Society for Horticultural Science
Place of publication
Leuven
Event location
Brisbane
Event date
Aug 17, 2014
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000391249900004