An inter-laboratory comparison of DNA barcoding protocols for identifying fungi involved in grapevine trunk disease

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F17%3A43912421" target="_blank" >RIV/62156489:43510/17:43912421 - isvavai.cz</a>

Result on the web

<a href="http://managtd.eu/images/uploads/content/257/Abstracts%2010th%20IWGTD%20Reims%202017.pdf" target="_blank" >http://managtd.eu/images/uploads/content/257/Abstracts%2010th%20IWGTD%20Reims%202017.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

An inter-laboratory comparison of DNA barcoding protocols for identifying fungi involved in grapevine trunk disease

Original language description

Many species of fungi are present in the grapevine trunk disease (GTD) complex. Accurate identification and characterisation of the causal agent or agents is essential for both successful diagnosis of trunk diseases and research into their epidemiology and control. Since identification based on morphological observations can be ambiguous due to similarity in colony characteristics many researchers rely on DNA sequencing based approaches to identify the fungal organism present. For fungi, the ribosomal DNA internal transcribed spacer (ITS) region has been adopted as the primary DNA barcode marker. However, despite its widespread use there has been relativity few studies comparing its use in routine fungal identification between laboratories. In this study, the utility of the ITS region as a means of accurately identifying GTD fungi was assessed in an international inter-laboratory comparison comprising of researchers participating in the EU cost action on GTD (manaGTD). Twelve samples of fungal DNA associated with GTD fungi were distributed to researchers in 14 laboratories. The researchers were asked to use their own DNA barcoding protocols to identify the GTD species using phylogenetic markers for the ITS, histone and elongation factor regions. The results and the relative robustness of DNA barcoding approaches for GTD fungi are discussed.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project

<a href="/en/project/LD14051" target="_blank" >LD14051: Development of a reliable system for detection and quantification of pathogens evoking GTD and GTD survey in the Czech wine regions</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů