Analysis of DNA for Differentiation of Fish Species in Seafood Samples

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F10%3A00002189" target="_blank" >RIV/62157124:16270/10:00002189 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Analysis of DNA for Differentiation of Fish Species in Seafood Samples
Original language description
Polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) analysis was used to identify fish species and to perform their authentication in commercial seafood products. The mitochondrial cytochrome b gene provides very low intraspecies variability, thus allowing the unequivocal identification of fish species. Universal primers were used for PCR amplification of 464-bp long fragments of this gene. The PCR products were digested with restriction enzymes AluI, HinfI, HaeIII, DdeI, NlaIII, HincII, MboII. Sixty fish samples were obtained from the local markets in the Czech Republic. At 47 samples (78.3%) the results were in agreement with declarations of the producers and 10 samples (16.7%) contained other fish species. There were notgreat differences between fresh fillets (chilled, frozen etc.) and heat processed foodstuffs (tinned, smoked, etc.). Correct designation was at 72.3% and 81.6% samples, respectively. Even if in 3 cases the analysis was unsuccessful the me
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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