A comparison of the Schade and Phadebas methods for determining diastase activity in honey

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F16%3A43876549" target="_blank" >RIV/62157124:16270/16:43876549 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

A comparison of the Schade and Phadebas methods for determining diastase activity in honey

Original language description

The aim of this work was to assess methods for determining diastase activity in honey. Eight samples of honey from the retail network and 48 blends of honey with syrups (Apifood and Apivital) were tested using the Schade method and the Phadebas enzyme method. One of the eight samples of honey failed to meet the requirements of the Decree No. 76/2003 Coll. Diastase activity values of 11.13 units according to Schade, though 7.61 Schade units according to the Phadebas method, were measured in this sample. The two methods produced different diastase activity values, with the values according to Phadebas being on average 12.48% lower. A statistically significant difference (p &lt; 0.05) was found between the methods. The cause of this difference in the results produced by the methods used was probably enzyme substráte specificity ? the specific properties of the starch used as a substrate. The Schade method can be recommended for testing a small number of samples, while the Phadebas method is more suitable for serial analyses.

Czech name

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Czech description

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Type

J_ost - Miscellaneous article in a specialist periodical

CEP classification

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OECD FORD branch

40401 - Agricultural biotechnology and food biotechnology

Project

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Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Maso International

ISSN

1805-5281

e-ISSN

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Volume of the periodical

6

Issue of the periodical within the volume

2

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

4

Pages from-to

119-122

UT code for WoS article

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EID of the result in the Scopus database

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